Table 1

Gene expression studies of breast carcinomas aiming to improve clinical management

Gene expression profiles and classifiers
Tissue/population included
Samples (n)
Platform
Number of differentially expressed cDNAs/oligos/probe sets (total)
Ref.

Overall outcome/metastasis
PBC (node -ve, age <55 years)
117
oligo Agilent Hu25k
70 (24,479)
[7]

PBC (node ±ve, age <53 years)
295
oligo Agilent Hu25k
70 (24,479)
[8]

PBC (node -ve, any age)
287
Affymetrix U133A
76 (22,283)
[9]

PBC (node -ve, ER+ only)
668
qRT-PCR
21 genes
[32,33]

PBC (population and validation cohorts)
448
Affymetrix U133 set
64 (44,792)
[37]
Tumour classification
PBC (+benign tissues)
65
cDNA (Stanford)
476 (8102)
[3]

PBC (+benign tissues)
78
cDNA (Stanford)
476 (8102)
[4]

PBC (+benign tissues)
122
cDNA (Stanford)
476 (8102)
[5]

PBC (node ±ve)
20
Agilent, Applied Biosystems, cDNA (Stanford)

[6]

PBC + metastases
105
Agilent oligo (1Av1, 1Av2)
1300 (>17,000)
[11]

PBC (node ±)
99
cDNA (NCI)
706 (7650)
[38]
ER status
PBC (2–5 cm, node -ve)
47
cDNA
100 (6728)
[39]

PBC (1.5–5 cm, node ±ve, ER/PR +ve or -ve)
49
Affymetrix HuGeneFL
100 (5600)
[40]

PRC (stage I-II, node -ve, ER and PR +ve)
26
SAGE
520 (>50,000)
[41]
Nodal status
PBC (ER ±ve, node ±ve)
49
Affymetrix U95A
100 (12,626)
[42]
Apocrine
PBC (large operable/advanced inoperable/inflammatory)
49
Affymetrix U133A
520 (22,283)
[10]
Wound Response
PBC (node ±ve, age <55 years)
295
Oligo Agilent Hu25k
442 (24,479)
[16]
Proliferation
PBC (node ±ve, age <55 years)
311
Oligo Agilent Hu25k
50 (24,479)
[17]
Grade
PBC (node ±ve, ER ±ve)
189
Affymetrix U133A
128 (22,283)
[12]
Grade/progression
Normal breast, ADH, DCIS, IDC (LCM)
61
cDNA (Research Genetics)
200 (1940)
[43]
Hereditary breast cancer
PBC (sporadic, BRCA1, BRCA2)
22
DNA clones
176 (6512)
[18]

Fibroblasts from normal breast (BRCA1, normal controls)
28
cDNA (IMAGE)
47 (5603)
[44]
Tamoxifen resistant
PBC (responsive and nonresponsive to tamoxifen)
112
cDNA (NKI)
81 (19,200)
[21]
ER response
MCF-7 cells and luminal breast tumours
65
cDNA (Agilent)
822 (>17,000)
[13]
Neoadjuvant chemotherapy response
PBC (docetaxel)
24
Affymetrix U95A
92 (12,626)
[20]

PBC (doxorubicin/cyclophosphamide)
40
Affymetrix U133A
253 (22,283)
[19]

PBC (doxorubicin)
36
Affymetrix U133A plus2.0
38 (54,678)
[14]

PBC (gemcitabine/epirubicin/docetaxel)
100
Custom made oligo
512 (21,329)
[45]

PBC (doxorubicin/cyclophosphamide)
16
cDNA (NCI)
137 (7650)
[46]

Whilst we have endeavoured to highlight the major studies in this field, we regret any offence to authors of other important work not included in this table. ADH, atypical ductal hyperplasia; DCIS, ductal carcinoma in situ; ER, oestrogen receptor; IDC, invasive ductal carcinoma; LCM, laser capture microdissection; PBC, primary breast carcinoma; PR, progesterone receptor; qRT-PCR, quantitative reverse transcription polymerase chain reaction.

Sims et al. Breast Cancer Research 2006 8:214   doi:10.1186/bcr1605