Figure 2.

Differential signal transducer and activator of transcription 3 (Stat3) phosphorylation in breast cancer-derived cell lines. Nuclear extracts (20 μg) isolated from MCF10A, MCF7, BT474, MDA-MB-435, MDA-MB-MD-468, and MDA-MB-MD-231 cell lines were analyzed by Western blotting with tyrosine-phosphorylated Stat3 (pStat3) and Stat3 antibodies.