Table 4 |
|||||
|
Summary of protocols and costs |
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|
Technical stage |
Stage presence or duration |
||||
|
|
|||||
|
PathVysion |
PharmDx |
INFORM |
CISH |
In-house |
|
|
|
|||||
|
Deparaffinisation and rehydration |
40 min |
33 min |
Overnight in the BenchMark |
25 min |
20 min |
|
Pretreatment |
67 min |
31 min |
21 min |
60 min |
|
|
Digestion |
26 min |
16 min |
16 min |
No |
|
|
Fixation |
20 min |
No |
No |
Fixation, blocking, incubation with primary and secondary antibodies and detection, all performed in NexES 90 min |
|
|
Dehydration |
No |
6 min |
10 min |
||
|
Drying |
Yes |
Yes |
Yes |
||
|
Denaturation |
5 min |
5 min |
5 min |
||
|
Hybridisation |
Overnight |
Overnight |
Overnight |
||
|
Washing |
4 min |
20 min |
4 min |
11 min |
Washing, haematoxylin counterstain (15 sec), dehydration and mounting, 12 min |
|
Dehydration |
No |
6 min |
3 min |
No |
|
|
Drying |
30 min |
Fast |
Fast |
No |
|
|
DAPI staining |
Yes |
Yes |
Yes |
No |
|
|
Detection |
No |
No |
No |
147 min |
|
|
Total duration |
Overnight + 3 h 12 min |
Overnight + 1 h 57 min |
Overnight + 7 min |
Overnight + 3 h 55 min |
3 h 2 min |
|
Price (€ per test) |
131 |
85 |
75 |
56 |
4 |
|
|
|||||
|
CISH, chromogenic in situ hybridisation; DAPI, 4,6-diamidino-2-phenylindole. |
|||||
|
Cayre et al. Breast Cancer Research 2007 9:R64 doi:10.1186/bcr1770 |
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