RGS2 overexpression attenuates oxytocin receptor signalling to p44/42 MAPK. (a) quantitative real-time PCR analysis of RGS2 gene expression in triplicate samples of stably transfected Hs578T-pcDNA (parental vector) compared with Hs578T-RGS2 cells. Mean expression levels ± 95% confidence limits are shown. Significant differences are indicated (**P < 0.01). RGS2 was fivefold overexpressed in the Hs578T-RGS2 cells compared with the control cell line. (b) Analysis of calcium flux in Hs578T-pcDNA and Hs578T-RGS2 cells stimulated with 5 × 10-7 mol/l oxytocin. Both cell lines showed an identical response to the stimulus. (c) Time course analysis of p44/42 mitogen-activated protein kinase (MAPK) phosphorylation in Hs578T-pcDNA and Hs578T-RGS2 cells stimulated with 5 × 10-7 mol/l oxytocin. (d) Quantitation of phosphorylation analysis. p44/42 MAPK phosphorylation was significantly reduced (P < 0.001) in the Hs578T-RGS2 cell line compared with the control Hs578T-pcDNA cells.
Smalley et al. Breast Cancer Research 2007 9:R85 doi:10.1186/bcr1834