RGS2 is expressed in human myoepithelial and luminal cells and in breast cancers. (a) RNA isolated from normal primary breast cells, normal and breast cancer cell lines and primary breast cancers was analysed by semi-quantitative RT-PCR for expression at the transcriptional level of RGS2, OXR and a housekeeping gene B2M. (b) Quantitative real-time PCR analysis of RGS2 expression levels in a selection of primary human cells, human breast cancer cell lines, solid breast cancers and F19-depleted cancers. Data are mean relative expression levels ± 95% confidence limits (n = 3 analyses of each sample). For comparison, the primary myoepithelial and primary luminal cell data from Figure 2c have been included on this graph. Note that the y-axis is a log10 scale.
Smalley et al. Breast Cancer Research 2007 9:R85 doi:10.1186/bcr1834